Analysis of the expression and functionality of GPR39 in ARPE-19 cells.

<p>(A) Immunocytochemical detection of GPR39 in ARPE-19 cells (objective magnification of 20x). (B) The effect of siRNA depletion of GPR39 on pAkt(S473) and pERK1/2(T202/Y204) in ARPE-19 cells after human obestatin treatment (<b>1</b>, 100 nM, 10 min). The ARPE-19 cells were transfected with GPR39 siRNA prior to obestatin <b>1</b> treatment. Equal amounts of protein in each sample were used to assess the expression of GPR39 by western blotting. The GPR39 level was expressed as the fold change relative to the control siRNA-transfected cells (mean ± SE). The protein expression was normalized relative to actin. The data are expressed as the mean ± SE. The asterisk (*) denotes <i>P</i><0.05 when comparing the treated control siRNA group with the control siRNA group; the dagger (#) denotes <i>P</i><0.05 when comparing the GPR39 siRNA group with the control siRNA group.