Inverse correlation between ING4 and p-p65/RelA (Ser276) antibody staining in breast tumor samples.

<p>(<b>A,B</b>) Hyperplastic breast tissue with moderate atypia from a ductal carcinoma in situ case, showing ING4 score +3 and p-p65 score 0. Inset is a higher magnification image of the region showing ING4 staining in luminal and basal epithelial cells and in stroma cells. (<b>C,D</b>) Invasive ductal carcinoma with ING4 score 0 and p-p65 score +2. (<b>E,F</b>) Grade 3 invasive ductal carcinoma with ING4 score +1 and p-p65 score +3. (bars, 100 µm). (<b>G</b>) Low ING4 expression is more prevalent in the tumors that express high levels of p-p65. Number of ING4-low (solid bars, IHC score <1.5) and ING4-high (slanted-line bars, IHC score ≥1.5) tumors, presented as a percentage of total number of tumors in each subgroup. Total (n = 227, 77 ING4-low, 150 ING4-high), ER+ (n = 156, 55 ING4-low, 101 ING4-high), ER− (n = 63, 18 ING4-low, 45 ING4-high), HER2- (n = 197, 67 ING4-low, 130 ING4-high), HER2+ (n = 27, 9 ING4-low, 18 ING4-high), p-p65-low (n = 166, 48 ING4-low, 118 ING4-high), and p-p65-high (n = 62, 29 ING4-low, 33 ING4-high). <i>P</i> values were determined by Fisher’s Exact test.