Neutralization of DENV-1 by E111 varies with time and temperature in a genotype-dependent manner.

<p>Serial dilutions of E111 were incubated with (<b>A</b> and <b>E</b>) DENV-1 16007, (<b>B</b> and <b>F</b>) West Pac-74, (<b>C</b> and <b>G</b>) V345A West Pac-74, or (<b>D</b> and <b>H</b>) A345V 16007 RVP for 1 hour at 37°C before the addition of Raji-DC-SIGNR cells to establish reference neutralization curves. Additional DENV-1 RVP-E111 complexes were incubated for 2, 4.5, 7, and 22 hours at 37°C (<b>A–D</b>) or 2 and 4.5 hours at 40°C (<b>E–H</b>) before addition to Raji-DC-SIGNR cells. Infection was carried out at 37°C and determined by flow cytometry 48 hours later. One representative experiment of three is shown. The data is normalized relative to the infectivity of the RVP in the absence of antibody at each time point for each temperature. Error bars indicate standard error of the mean of replicate infections.