Cytoskeletal organization changes as hydrogel rigidity is altered.

<p>Representative fluorescent images of NIH3T3 cells stained for F-actin (red), focal adhesion protein vinculin (green), and nuclear DNA (blue, although appears slightly purple due to the actin colocalization). Panels A–C show cell patterns obtained on different stiffness regions on the same hydrogel. Panel D shows cells on glass coated with fibronectin.