Immunohistochemical analysis of CD20-negative/CD3-negative cutaneous and muscle-associated lymphomas from rhesus and cynomolgus macaques.

<p>CD20-negative/CD3-negative lymphomas from a SHIV-positive female cynomolgus macaque (93034; cutaneous) (A,B) and a SIV-positive male rhesus macaque (A01112; abdominal muscle-associated lymphoma)(C,D) were analyzed by immunostaining: A) anti-gB (1508)(RV2) staining is evident in the entire cutaneous tumor of 93034 surrounded by displaced skin; B) High magnification of the region in A circled in black shows widespread expression of gB in the perinuclear area and cytoplasm; C) anti-ORF59 staining is evident in the majority of lymphoma cells in the A01112 lymphoma. High magnification of the boxed region shows strong nuclear, perinuclear and cytoplasmic ORF59 staining (insert); D) anti-gB (1508)(RV2) staining is evident in the majority of lymphoma cells in the A01112 lymphoma. High magnification of the boxed region shows strong staining in the cytoplasm and perinuclear regions, with an aggregate morphology. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002962#ppat-1002962-g007" target="_blank">Figures 7A and C</a> were autoleveled using Adobe Photoshop. Scale bars = 1 mm (A); 75 µm (B); 37 µm (C, D).