<i>In vivo</i> characterization of neural crest derived cells.

<p>To characterize neural crest-derived clones <i>in vivo</i>, we stereotaxically injected 50,000 cells of each NCSC clones (separetly) in mice striatum (<b>A</b>). <i>Asclepios</i> induced massive tumors after 4 weeks as attested by the beta-galactosidase expression of the tumor cells. (<b>B</b>). Immunological characterization of those tumors revealed that they were GFAP (<b>c</b>), beta-III-tubulin (<b>D</b>), nestin (<b>E</b>), N-cadherin (<b>F</b>) and NrCAM-positive (G). Lectin labeling (<b>H</b>) confirmed the presence of blood vessels in the tumors. Finally, no vimentin (<b>I</b>) or Sox2 (<b>J</b>) expressions were observed. Nuclei were counterstained with Dapi (blue). Scale bars = 50 µm.