CGK062 represses the expression of the β-catenin-dependent genes.

<p>(A) PC3, SNU475, and SW480 cells were co-transfected with cyclin D1-RL and pSV40-FL, and then incubated with indicated amounts of CGK062 for 15 h. Luciferase activities were measured 39 h after transfection. Cyclin D1 promoter activity is reported as relative light unit (RLU) normalized to firefly luciferase activity. The results are the average of three experiments, and the bars indicate standard deviations. *, <i>P</i><0.05, and **, <i>P</i><0.01, compared with the vehicle control group. (B) PC3, SNU475, and SW480 cells were incubated with the vehicle (DMSO) or CGK062 for 15 h and then cell extracts were prepared for Western blotting with anti-axin, anti-cyclin D1 and anti-myc antibodies. To confirm equal loading, the blots were reprobed with anti-actin antibody.