Transcription factor activity and phospho-Akt in cells after overexpression of TFPI.

<p>(A) Transcription factor activity was measured using the cignal finder luciferase reporter system. SK-BR-3 cells (3×10⁴) transiently transfected with vectors overexpressing TFPIα (dark gray) or TFPIβ (gray), or with empty vector pTOPO (light gray) as controls were seeded in 96-well arrays 24 hours after transfection. After 48 hours, cells were lysed and the firefly and renilla luciferase intensity determined. The results are presented as mean (n≥8) relative luciferase activity + SEM of three independent experiments. Statistical differences between cells overexpressing TFPI and empty vector control cells were determined using the student's t test (* <i>p</i><.05, ** <i>p</i><.01, *** <i>p</i><.001, ns = not significant). (B) Western blot of phospho-Akt in SK-BR-3 cells overexpressing TFPI. Band intensities were measured using ImageJ and the intensities of phospho-Akt were normalized to Akt levels.