Endogenous BR levels in <i>35S:PIZ</i> and <i>35S:PIZ bri1-301</i> seedlings.

<p>(<b>A</b>) Free BR levels in aerial tissues of 4-week-old <i>35S:PIZ</i> seedlings quantified by GC-MS are shown in ng/g fresh weight (FW). 6-deoxo-cathasterone (6-deoxoCT), 6-deoxo-teasterone (6-deoxoTE), 3-dehydro-6-deoxoteasterone (6-deoxoDT), 6-deoxo-typhasterol (6-deoxo-TY), 6-deoxo-castasterone (6-deoxoCS), cathasterone (CT), teasterone (TE), 3-dehydro -teasterone (3DT), typhasterole (TY), castasterone (CS), brassinolide (BL). n.d., not detected. (<b>B</b>) Growth phenotypes of 17-day-old Col-0, <i>bri1-301, 35S:PIZ</i> and <i>bri1-301 35S:PIZ</i> (from left to right). Scale bar represents 3 cm. (<b>C</b>) Free BL and CS levels from aerial tissues of 17-day-old plants are analysed by LC-ESI-MS/MS. Mean values of three biological replicates are shown, relative to fresh plant weight (FW), with SD.