EGFR protein levels detected in the lysate of transfected SK-BR-3 cells by Western blot analysis.

<p>Proteins were separated on a SDS-polyacrylamide gel, transferred to a nitrocellulose membrane and detected using an anti-EGFR antibody. Anti-actin was used as a protein loading control. (A) Western blot of one representative experiment. (B) Quantification of three independent experiments using ImageJ (n = 3+ SEM).