Dependence of Cpl-7 CD spectra with temperature at neutral pH.

<p>(<b>A</b>) and (<b>B</b>) show the spectroscopic changes induced by the temperature increase in the far- and near-UV regions, respectively. Isodichroic points in the far-UV region are marked as “a” and “b”, and the T arrow indicates the temperature increase (20, 30, 40, 45, 50, 55, 60, 65, 70, 85 and 95°C). The near-UV spectra correspond to 20°C (black), 50°C (green), 55°C (blue), 65°C (red) and 95°C (magenta). (<b>C</b>) compares the thermal denaturation profiles of Cpl-7 at 195 nm (circles), 203 nm (triangles), 208 nm (diamonds) and 290 nm (hexagons). Measurements were performed in 20 mM Pi buffer, pH 7.0, at heating rates of 20°C h⁻¹ (solid symbols) and 40°C h⁻¹ (open symbols).