VASH1 inhibits premature senescence and cell death of HAECs.

<p>(A) HAECs were transfected with VASH1 siRNA or control siRNA. After a 24-hour incubation, RT-PCR and Western blotting for VASH1 were performed. (B) After a 6-day incubation, SA beta-gal staining was performed on HAECs that had been transfected with VASH1 siRNA or control siRNA. SA beta-gal-positive HAECs were quantified, and the % of senescent cells was calculated (*P<0.01, N = 3). (C) HAECs were infected with AdVASH1 or control AdLacZ. After a 24-hour incubation, RT-PCR and Western blotting for VASH1 were then performed. (D) HAECs infected with AdVASH1 or control AdLacZ were exposed to 100 µmol/L H₂O₂ or to 0% FCS/DMEM for 24 hours. The trypan blue exclusion assay was performed to judge cell death (*P<0.01, N = 3). All the studies were repeated at least 3 times to confirm the reproducibility.