Protein-protein bio-conjugation of GFPhs-r5M-Hpg and GFPhs-r5M-Aha.

<p>(A) Copper (I)-catalyzed cycloaddition (CCCA) reaction between azide and alkyne incorporated to GFPhs-r5M resulted in the formation of triazole-linked protein-protein dimer bio-conjugation. (B) SDS-PAGE analysis of CCCA reaction between GFPhs-r5M proteins incorporated with Hpg (alkyne) and Aha (azide group). Lane 1: CCCA reaction without catalysis agents, CuSO₄ and L-ascorbic acid; lane 2: CCCA reaction with catalysis agents, CuSO₄ and L-ascorbic acid. This result shows the formation of triazole-linked protein-protein bio-conjugation of GFPhs-r5M dimer. M is molecular weight marker, thick arrow indicates the protein-protein conjugated GFPhs-r5M dimer of 55.2 kDa and grey arrow indicates the 27.6 kDa monomer of GFPhs-r5M containing Hpg and Aha respectively.