Role of TS mRNA synthesis in TS protein up-regulation.

<p>The concentration of TS protein in 2008 (panel A) and C13* cells (panel B) was determined by Western blotting at various times after the addition of 5-FU to the control media (closed squares) and DRB (inhibitor of RNA pol II)-treated cells (closed triangles). For each time selected 10 µg of protein extract was resolved in SDS-PAGE (12%) and the TS protein amount is given with respect to time 0. Results represent the mean of five separate experiments. Red Ponceau staining of the membranes prior to immune-detection was used as loading control <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047318#pone.0047318-RomeroCalvo1" target="_blank">[66]</a> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047318#pone.0047318.s003" target="_blank">Figure S3</a>). Immunodetection of typical experiment is shown in the bottom part of each panel. Statistical significance was estimated by two-tailed with unequal variance Student's t-test comparing samples treated with 5-FU with samples treated with 5-FU+DRB at each selected time (*P<0.05; **P<0.01, n = 5). Error bars indicate S.D.