Time course of tumor growth and metastasis.

<p><b>A and B</b>, Nude mice were inoculated with either 5×10⁶ SK-Hep-1 (A) or SK-GI-18 cells (B), randomized into groups and animals were treated by daily gavage starting on day 4 with vehicle, Px20606 (10 mg/kg/d) or Sorafenib (100 mg/kg/d) as indicated. <i>In vivo</i> imaging pictures for representative animals from each group that received SK-Hep-1 (A) or SK-GI-18 (B) cells at the indicated days are displayed. <b>C</b>, Time course of tumor development in the liver. The <i>in vivo</i> fluorescence intensities at day 7 were set to 100% for each group and data plotted for data gathered at days 7, 14, 21, 28, 35 and 56. The sharp rise of fluorescence in the SK-Hep-1/Px20606 group from day 35 to day 56 is due to sharp increases in tumor sizes in just three individual animals out of a total of nine animals (fluorescence data from these animals on day 56 are circled in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043044#pone-0043044-g006" target="_blank">Fig. 6A</a>).