α-MV antibody-negative serum does not neutralize MV_NSe-LV or targeting vectors.

<p>Equal amounts of physical particles of the indicated vector types were incubated in serial serum dilutions of an α-MV antibody-negative donor. Then, (<b>a</b>) 1×10⁴ (CD20-LV) and 2×10⁵ (MV_NSe-LV) CD20-positive Raji cells were added, respectively, or the dilutions were added to (<b>b</b>) CD133-positive HuH7 cells. These were seeded at a density of 1.0×10⁴ (targeting vectors) and 5.0×10⁴ (MV_NSe-LV) cells per 96 or 48 well, respectively, 24 h before transduction, to apply similar MOIs of vector particles. Forty-eight to 72 h later, the percentage of EGFP-positive cells was determined by FACS analysis. As control, medium without serum was used.