Thermal stability of prion polymers of full-size and truncated Sup35.
- Alexander I. Alexandrov (128845)
- Alla B. Polyanskaya (128846)
- Genrikh V. Serpionov (128847)
- Michael D. Ter-Avanesyan (128849)
- Vitaly V. Kushnirov (128851)
Publication date
October 2012
DOIAbstract
<p>(A) The lysates of [<i>PSI</i><sup>+</sup>] yeast cells producing full-sized or truncated Sup35 were analyzed by SDD-AGE. (B, C) Lysates were heated in the presence of sample buffer containing 2% SDS at different temperatures and analyzed by SDD-AGE. The thermal denaturation curves were derived from densitometric analysis of the stained blot images.</p