The fit of our models to the observed steady-state values determined experimentally.

<p>Different antibody assembly models were fitted to the different observed steady-state data given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047422#pone-0047422-t001" target="_blank">Table 1</a>. Panels on the left-hand side (A and C) show the assembly routes for the two different models, with productive reactions (those leading to the formation of full antibody) displayed in red. The panels on the right-hand side (B and D) indicate the difference between the best-fitting parameter set in the biochemical models and the observed parameter values. The two models, as well as a model combining the two assembly pathways, can all reproduce the experimentally observed data for all four cell-lines with high similarity. Similar results were obtained for the engineering models. H.mRNA (heavy chain mRNA), L.mRNA (light chain mRNA), LHHL (assembled antibody), LH (half antibody species), H (heavy chain), HH (heavy chain dimer), HHL (heavy chain dimer +1 light chain), LL (light chain dimer), L (light Chain), mAb (assembled secreted antibody), qmAb (mAb secretion rate), qHL (HL secretion rate), qLL (LL secretion rate), qL (L secretion rate).