The effect of pH on the fluorescence emission and absorbance of Rtms5^Y67F, Rtms5^Y67F/H146S, Rtms5 and Rtms5^H146S.

<p>Absorbance and fluorescence emission spectra were determined at different pH in buffers of constant ionic strength for (A), Rtms5^Y67F; (B), Rtms5^Y67F/H146S; (C), Rtms5 and (D), Rtms5^H146S. Values shown are those at the and for each protein. Excitation was 440 nm for A and B, and 590 nm for C and D.