Down-regulation of <i>miR-101</i> in breast cancer tissues.

<p>We pooled two patients' cancer tissues as a sample. The expression level of <i>miR-101</i> in individual sample was detected by TaqMan miRNA RT-Real Time PCR (A). Statistical analyses were performed to analyze the overall trend of <i>miR-101</i> in all human breast cancer tissues (B), ER/PR−,HER2− (C), ER/PR+, HER2+ (D), ER/PR+, HER2− (E) and ER/PR−, HER2+ (F) breast cancer tissues. Relative expression of <i>miR-101</i> in breast cancer tissues and adjacent normal breast tissues was compared to U6 in corresponding tissue sample, i.e. the relative level of <i>miR-101</i> was normalized to U6. U6 serves as an internal reference among different samples and helps normalize for experimental error. The <i>y</i>-axis displays the relative log2 ratio of <i>miR-101</i> normalized by <i>U6</i>. *<i>P</i><0.05, **<i>P</i><0.01.