SUP-12 represses excision of intron 1A from the endogenous <i>unc-60</i> transcript.

<p>(<i>A</i>–<i>D</i>) RT-PCR analyses of mature mRNAs (<i>A</i>) and partially spliced RNAs (<i>B</i>–<i>D</i>) from the endogenous <i>unc-60</i> gene. Total RNAs from synchronized L1 larvae of N2 (lanes 1 and 3) and <i>sup-12 (yb1253)</i> mutant (lanes 2 and 4) were subjected to RT-PCR without (lanes 1 and 2) or with (lanes 3 and 4) reverse transcriptase (RT). Positions of the primers are indicated on the left. Each band is numbered in the order of size. Schematic structures of the RNAs are indicated on the right. Black and blue triangles indicate positions and directions of exonic and intronic primers, respectively. Asterisks denote artificially amplified fragments.