The effects of <i>miR-101</i> on cell migration of breast cells.

<p>T47D, MDA-MB-231, MCF-7 and Hs518bst cells were transfected with pre-miR control, <i>miR-101</i> mimics, anti-miR control or <i>miR-101</i> inhibitor, respectively. Cells were harvested 48 h after transfection and recounted to 0.5×10⁶ cells/ml in every group to seed Transwells for cell migration assay. At time of harvest, the cells on top of the membranes were removed, and the cells on the bottoms of the membranes were stained with haematoxylin and eosin. The cell migration was quantified by counting the amount of cells passing through the membrane from five different fields per sample at 100× selected in a random manner after 17 h of incubation. A show the representative photomicrographs of cells passing through the membrane at ×100 original magnification. Histogram B-E represents the number of migrated cells in MCF-7 MDA-MB-231, T47D and Hs518bst cells, respectively. Data are expressed as the mean numbers of independent triplicate experiments. *<i>P</i><0.05.