Decreased responsiveness to hypoacylated LPS by BMDM from humanized TLR4/MD-2 mice.

<p>(A) BMDM (CD11b+ F4/80+ cells) were stained for surface TLR4 expression at the time of harvest, prior to stimulation. Blue histograms depict anti-mouse TLR4/MD-2 staining, red histograms anti-human TLR4/MD-2 and filled grey histograms isotype controls. Shown is one representative experiment of 2. (B) BMDM from the indicated mice were stimulated with increasing concentrations of LPS for 4 hr and cytokines/chemokines were measured in the supernatant by Luminex. Shown are the mean and range of 2 independent experiments (one well per stimulation per experiment).