Acute effects of LPS treatment on SCN.

<p>Animals were treated with either saline or LPS and 24 hours later sampled. There was no increase in the markers for apoptosis TUNEL and cleaved-caspase 3 (A; n = 4–5 for the saline and acute LPS groups), there was a significant increase in EGR-1 but not ARC (B; n = 5 for the saline and acute LPS groups), and whilst F4/80 and NOS2 immunoreactivity in the SCN was significantly elevated in the LPS-treated animals, TNF-α was not (n = 4–5 for the saline and acute LPS groups). *** P<0.001, ** P<0.01, * P<0.05.