The conidiation defects of <i>cchA</i>/<i>midA</i> mutants can be rescued by extra-cellular Ca²⁺ and osmotic or cation stress.

<p>(A) Conidiation defects of <i>cchA</i>/<i>midA</i> deletion mutants can be rescued by extra-cellular Ca²⁺. The indicated strains were inoculated onto MMPDR with 2 µl of 10⁹ conidia ml⁻¹ supplemented with different doses of CaCl₂ as indicated. (B) Conidiation defects of <i>cchA</i>/<i>midA</i> deletion mutants can be rescued by osmotic stress or ion stress. 2 µl of 10⁹ conidia ml⁻¹ were spotted onto MMPDR supplemented with 800 mM NaCl, 600 mM KCl, 1 M Sorbitol, 1 M Sucrose, 200 mM LiCl respectively. (C) Conidiation defects of <i>cchA</i>/<i>midA</i> deletion mutants can be rescued by extra-cellular Ca²⁺ in calcineurin-dependent way. 2 µl of 10⁹ conidia ml⁻¹ were spotted onto MMPDR supplemented with 40 mM CaCl₂, 1 µM FK506 and 40 mM CaCl₂ plus 1 µM FK506 respectively. (D) Conidiation defects of <i>cchA</i>/<i>midA</i> deletion mutants can be rescued by osmotic stress in a calcineurin-indenpendent way. 2 µl of 10⁹ conidia ml⁻¹ were spotted onto MMPDR supplemented with 800 mM NaCl, 1 µM FK506 and 800 mM NaCl plus 1 µM FK506 respectively. All plates were incubated at 37°C for 48 h.