Visualising chromatin remodeling during particle displacement.

<p>(a) Spatial map of fluorescence anisotropy of the chromatin (H2B-EGFP) assembly before and after movement of the bead through the nucleus. The black dots represent the trajectory of the bead and the start point is marked by the white arrow. Scale bar m. (b) Time course of anisotropy maps of chromatin on application of force on a m paramagnetic bead in the nucleus. Arrows indicate the initial position of the bead. (c) Histogram of anisotropy of pixels visited by the beads in the nuclei () and anisotropy of pixels not visited by the beads as extracted from anisotropy image acquired before bead movement.