Akt mediated the potentiating effect of PIK-75 on the glucose-induced secretion.

<p>Cultured islets transfected with PKCζ(ΔPS) (A; n = 6 for each group), myr-Akt1 (D; n = 6 for each group) and PDK1(A280V) (H; n = 6 for each group) pretreated with or without 0.5 µM PIK-75 or 1 µM Akti-1/2 for 30 min were stimulated with 16 mM glucose. Alternatively, insulin secretion from cultured islets pretreated with PKCζ-PS (B; n = 8 for each group) or Akti-1/2 (F; n = 8 for each group) for 30 min were stimulated with 16 mM glucose. COS7 cells transfected with myr-Akt1 (C) or PDK1(A280V) (G) were treated with or without 0.5 µM PIK-75 or 1 µM Akti-1/2 for 30 min and subjected to immunoblotting using anti-Pi-GSK3β and α-tubulin antibodies. (E) Cultured islets pretreated with Akti-1/2 for 30 min were subjected to immunoblotting using anti-Pi-GSK3β and α-tubulin antibodies. Data are represented as a mean ± S.E.M. *; p<0.03, **; p<0.01; N.S.; p>0.3.