Effect of HFD on the adiposity, the plasma and fecal LPS contents, and the proinflammatory cytokines in TLR4 deficient mice.

<p>(A) Body weight (g) was measured. (B) Epididymal fat pad (% of body weight) was calculated. LAL assay was used to measure (C) fecal endotoxin concentration (EU/g feces) and (D) plasma endotoxin concentration (EU/mL). (E) Concentrations of proinflammatory cytokines (pg/mL) in the plasma (left) and colon (right) were measured by ELISA. (F) mRNA expression levels in the adipose tissue were measured using real-time PCR. All values were indicated as the mean ± SEM (n = 5). **, <i>p</i><0.01 compared with LFD.