Overexpression of STING induced IFN and ISG expression.

<p>EPC cells were transfected with 2 µg of pcDNA vector encoding STING, or MAVS as a positive control, or an empty vector (pcDNA) as a negative control. At 48 h posttransfection, EPC were infected or not with VHSV at an MOI of 1 and incubated at 15°C for 24 h before total RNA extraction. Quantitative Real-time RT-PCR was conducted using primers targeting IFN (A), RIG-I (B) and Viperin (C). The β-actin gene was used as an internal control to normalize the cDNA template and to do real-time PCR calculations. SD of triplicate experiments has been calculated. Asterisks indicate significant difference (*p<0.05, **p<0.01 and ***p<0.001) as determined by Student’s <i>t</i> test.