Identification of <i>Nmnat2</i> mutants and transposon localization.

<p>Images of E18.5 embryos (A, B). In both images, the wild-type (WT) is on the left and <i>Nmnat2^blad/blad</i> (BLAD) on the right. (C) Diagram showing the localization of the pT2. BART3 transposon between exons 1 and 2 in the <i>Nmnat2</i> gene on chromosome 1. The solid arrows labeled <b>A</b> and <b>B</b> indicate the primer pair used for identification of the native gene, and the solid forward arrow <b>A</b> is combined with the hollow reverse arrow <b>C</b> to identify the presence of the transposon. IR/DR, inverted repeat/direct repeat; S.A., splice acceptor sites (Green/Blue); red hexagon, 3X stop codons; pA, synthetic Poly A signal. (D) PCR results using the primers shown in (C).