E2F6 repression of the E2F1 promoter is hindered by a dominant negative BRG1.

<p>Transfections were performed using T98G cells plated onto 6-well dishes. All wells were co-transfected with a construct expressing Lac Z and a E2F1 reporter plasmid in addition to the indicated plasmids. Luciferase activity was determined from triplicate samples and expressed as the average relative luciferase units after normalization to β-galactosidase activity. A) Increasing concentrations of E2F6 is correlated with a decrease in E2F1 reporter activity. A plasmid construct expressing E2F6 was transfected into T98G cells in 6 well dishes at the indicated amounts. The total mass of transfected DNA in each well was kept constant by adding empty vector plasmid DNA, when necessary. B) The ability of E2F6 to downregulate E2F1 promoter activity is inhibited by the presence of a dominant negative BRG1. A plasmid expressing a dominant negative BRG1 or an empty vector control was co-transfected with a plasmid construct expressing E2F6 into T98G cells. Luciferase activity was determined as described above.