Fork recovery by homologous recombination results in replication slippage.

<p>A. Serial tenfold-dilutions from indicated strains (<i>t-ura4-dup20-ori</i> associated or not with the <i>RTS1</i>-RFB) were spotted onto the indicated media after cell growth with (Rtf1 -, repressed) or without (Rtf1 +, expressed) thiamine. <i>RTS1</i>-RFB activity is given for each construct and condition. B. Frequency of Ura⁺ revertants in indicated strains after cell growth with (Rtf1 repressed) or without (Rtf1 expressed) thiamine. The <i>RTS1</i>-RFB activity is given for each construct and condition. Values correspond to the mean of at least three independent experiments and error bars correspond to the standard error of the mean (SEM). C. Rate of replication slippage in the indicated strains and conditions. The rate of Ura⁺ revertants was calculated using the method of the median from at least 11 independent cultures. Values in brackets indicate the 95% confidence interval. Statistical significance was detected using the nonparametric Mann-Whitney U test. D. Serial tenfold-dilutions from the strains indicated spotted onto the media indicated after cell growth without thiamine. <i>RTS1</i>-RFB activity “–” refers to the strain <i>t-ura4-dup20-ori</i> and “+” refers to the strain <i>t-ura4-dup20. E. Kinetics of Ura⁺ revertants frequency for the strains indicated as a function of the number of generations after thiamine removal. <i>RTS1</i>-RFB activity “–” refers to the strain <i>t-ura4-dup20-ori</i> and “+” refers to the strain <i>t-ura4-dup20. The values reported are the means of two experiments. F. The rate of replication slippage/generation for the strains indicated with (<i>t-ura4-dup20) or without (<i>t-ura4-dup20-ori</i>) the active <i>RTS1</i>-RFB. The rate was calculated from the slope of the curves presented in panel F. The values reported are means of three independent experiments and error bars correspond to SE.</i></i></i>