<i>In silico</i> measurements of the mutant cells in the G2-M model.

a<p>Each mutation is considered to have mainly functional (F) or structural (F) impact according to their locations in its target protein.</p>b<p>ΔΔG of the mutations in individual Cdk1 or CycB; each of them is an average value considering structures sampled from molecular dynamic simulations.</p>c<p>ΔΔG of the mutations in Cdk1-CycB complex (MPF); each of them is an average value considering structures sampled from molecular dynamic simulations.</p>d<p>Maximum of ΔΔG considering both complexed and uncomplexed states of the target protein.</p>e<p>Perturbation on CycB degradation was weighted 0.3 for the degradation of monomeric CycB and weighted 0.7 for the degradation of complexed CycB (MPF).</p>f<p>Perturbation on Cdk1 degradation was estimated through the degradation of MPF only since the amount of total Cdk1 is constant.</p>g<p>Perturbation on the interaction between CycB and Cdk1 was estimated through J_wee and J₂₅ with a weighting 0.9*J_wee+0.1*J₂₅.</p>h<p>The high ΔΔG is a result of van der Waals clashes when the target residue is mutated to a larger side chain.