Effect of transient activation of β-AR on cytosolic [cAMP] in RASMCs.

<p>Cytosolic cAMP measurements were conducted in cultured RAMSCs cells using the FRET-based cAMP sensor Epac1-camps in response to a short application of isoproterenol (Iso, 0.1 µM, 15 s). <b><i>A</i></b>: Representative cAMP signals monitored in one cell. Pseudocolor images reflecting the CFP/YFP ratio were recorded at the times indicated by the letters on the graph. <b><i>B</i></b>: Dynamic parameters (peak amplitude, t_max, t_1/2on, and t_1/2off) of isoproterenol-induced FRET signal as shown in <i>A</i>. Data are mean±SEM of 124 independent cells.