Effect of transient activation of β-AR on submembrane [cAMP] in RASMCs.

<p>Submembrane cAMP measurements were conducted in cultured RASMCs cells using the plasma membrane-targeted FRET-based cAMP sensor pm-Epac2-camps in response to a short application of isoproterenol (Iso, 0.01 µM, 15 s). <b><i>A</i></b>: Submembrane localization of pm-Epac2-camps was ascertained by recording the CFP emission. <b><i>B</i></b>: Variation of the CFP/YFP ratio monitored in one cell. <b><i>C</i></b>: Dynamic parameters (peak amplitude, t_max, t_1/2on, and t_1/2off) of isoproterenol-induced FRET signal as shown in <i>B</i>. Data are mean±SEM of 23 independent cells.