Effect of PDE inhibition on basal submembrane cAMP signal in RASMCs.

<p>Submembrane cAMP measurements were conducted in cultured RASMCs cells using the FRET-based cAMP sensor pm-Epac2-camps. The % increase in CFP/YFP ratio induced by the different PDE inhibitors or the vehicle was calculated after a 3 min-incubation. Data are mean±SEM of 8–14 independent cells as indicated. *** P<0.001 <i>versus</i> vehicle; ^## P<0.01 <i>versus</i> Cil and <i>versus</i> Ro.