WNT10A promotes RCC cell migration and invasion in transwell assay.

<p>Transwell assay was used to evaluate the effects of WNT10A on cell migration. After 48 h of transfection, 1×10⁴ cells were transferred into 8-µm inserts and incubated for another 48 h. Forced WNT10A expression in 786-O significantly increased the migration ability of these cells through the transwell; however, cotransfection with β-catenin siRNA reduced the WNT10A promotive effect on cell migration. Conversely, WNT10A siRNA knockdown in Caki-1 significantly reduced the migration ability of these cells. Bar charts show the cell migration ratio; significances were analyzed using the Student’s <i>t</i>-test. Invasion assay took advantage of the Matrigel-coated transwell assay. After 48 h of transfection, 2×10⁴ cells were transferred into 1 mg/mL Matrigel-coated insert and incubated for another 48 h. Forced WNT10A expression in 786-O significantly increased the invasive ability of these cells, but cotransfection with β-catenin siRNA reduced the WNT10A promotive effect on cell invasion. Conversely, WNT10A siRNA knockdown in Caki-1 significantly reduced the invasive ability of these cells. Bar charts show the cell invasion ratio; and significances were analyzed as mentioned above.