Bar-p38 kinase activation was barnacle lifecycle dependent.

<p>(A) pp38 was highly expressed in the cyprid stage. Equal amounts of extracts (60 µg) from NIII-IV, NV-VI, cyprids and juveniles at Day 1 were prepared and blotted with anti-pp38 and anti-total p38 antibodies, respectively. A representative of three experiments is shown. (B) Ratio of pp38 to total p38. The ratio of pp38 to total p38 for NIII-IV represents 1 unit. NIII-IV: nauplii III-IV; NV-VI: nauplii: V-VI. a,b,c, means with different letters are significantly different, P<0.001.