Molecular overview of the nematode families and genera monitored in our study.

<p>Specificity of primer combinations is expressed as the gap between the C_t value of the latest target and the C_t value of the earliest non-target (ΔC_t expressed in number of PCR cycles). For relationship between C_t value and number of target nematodes see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047555#pone-0047555-g004" target="_blank">Fig. 4</a>, and Materials and Methods in ‘<i>Relationships between C_t values and numbers of target nematodes</i>’.</p><p>B: bacterivore, F: fungivore, FP: facultative plant parasite (only for nematodes where this guild occurred in combination with fungivory), O: omnivore, P: predator; N/A: no quantitative PCR signal produced by non-target(s); ^*: number of genera within one family assessed by qPCR (families as in De Ley <i>et al</i>. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047555#pone.0047555-DeLey1" target="_blank">[66]</a>); **: as <i>Mylonchulus</i> is expected to occur in this area, its family has been included as additional taxon.