miR-200f and EMT-transcriptional inducers are modulated during <i>in vitro</i> EMT in breast basal cell lines.

<p>Expression of (<b>A</b>) miR-200f and (<b>B</b>) EMT-transcriptional inducers was evaluated by qRT-PCR in the sorted epithelial (EpCAM+) and mesenchymal (Fibros) subpopulations within MCF12A and Myo1089 cell lines. Data are normalized to the expression of <i>RNU48</i> and <i>18S</i> respectively. Bars represent mean expression changes ±SE in Fibros subpopulation relative to EpCAM+ cells (baseline). Three biological replicates were measured. Moderated t-test was performed to evaluate statistical significance (<i>p</i><0.001 for all miRNAs analyzed in panel A. <i>NS</i>, non significant). (<b>C</b>) Methylation status of <i>miR-200f</i> promoter sequences in EpCAM+ and Fibros subpopulations within MCF12A and Myo1089 cell lines. Histogram bars represent averaged methylation levels (±SE) for the promoter sequences of <i>miR-200f loci</i>. Statistically significant differences are indicated (*<i>p</i><0.001, Student’s t-test).