Reduced UPR levels and gene expression in ATF6α−/− mice after MPTP/P injections.

<p>Protein expression of neurotrophic factor (A I), anti-oxidative genes (B I), astrogliosis-inducing factor (C I, II), and the UPR-target genes (A I, B I). Protein extracts from brains (CPu) of wild-type or ATF6α −/− mice that were injected or not injected with MPTP/P were subjected to Western blotting with the indicated antibodies (A I, B I, C I), or subjected to IL-6 ELISA (C II). Relative intensities are shown in the graphs. The intensity of the genes from wild-type mice without MPTP/P administration is designated as one. Values shown are the mean ± S.D. *P<0.05, compared with mice without MPTP/P administration. ^#P<0.05, compared between wild-type and ATF6α −/− brains (n = 4). Transcripts of neurotrophic factor (A II), anti-oxidative genes (B II), astrogliosis-inducing factors (C III), and the UPR-target genes (A II, B II). Total RNA (1µg) isolated from wild-type or ATF6α −/− brains (CPu) at indicated times after 1^st MPTP/P injection was subjected to qRT-PCR with specific primers for the indicated genes. The relative intensity of the genes from wild-type mice not administered MPTP/P is designated as one. Values shown are the mean ± S.D. *P<0.05, **P<0.01, compared with the mice without MPTP/P administration. ^#P<0.05, compared between wild-type and ATF6α −/− brains (n = 4).