Epithelial expression of Gli2 in the <i>Gast^−/−</i> antrum.

<p>The antral expression of Hedgehog pathway molecules was determined in 9–13 month-old littermate controls (<i>Gast^+/+</i>) (panels <b>A</b>, <b>C</b> and <b>E</b>) and <i>Gast^−/−</i> (panels <b>B</b>, <b>D</b> and <b>F</b>) mice by X-gal staining of LacZ reporter mice for Sonic hedgehog (<i>Shh</i>) (<b>A</b> and <b>B</b>), <i>Gli1</i> (<b>C</b> and <b>D</b>) and <i>Gli2</i> (<b>E</b> and <b>F</b>). A high power field of Gli2-LacZ staining is shown in F, where nuclear (yellow arrows) and perinuclear (black arrows) staining was observed along with cytoplasmic reporter accumulation. Whole stomachs from <i>Gast^+/+</i> and <i>Gast^−/−</i> were analyzed for gene expression of <i>Shh</i> (<b>G</b>), <i>Gli1</i> (<b>H</b>), and <i>Gli2</i> (<b>I</b>). Bars in panels <b>A</b> to <b>F</b> are 100 µm. Data presented as mean±SEM. N = 8 per group. *P≤0.05.