Identification of O-GlcNAc bearing proteins purified from soleus skinned biopsies.

<p>(A) corresponds to a silver stained SDS-PAGE of O-GlcNAc proteins purified by RL-2 immunoprecipitation before (a) or after (b) an hexosaminidase treatment; (c) corresponds to immunoprecipitation protocol realized without proteins sample, and (d) to 25 µg of contractile proteins extract. O-GlcNAc proteins were purified by RL-2 immuno-precipitation followed by an electrophoretic separation on 7.5% (B) or 10–20% (C) SDS-PAGE. Gels were colloïdal blue stained. Each well-resolved bands were submitted to a mass spectrometry-based identification. Numbers on the two panels indicates the different identified proteins; note that the corresponding identifications are summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048218#pone-0048218-t003" target="_blank">table 3</a>.