Altered vascular gene expression and endothelial proliferation in class II <i>orc3/hGFAP-cre</i> mutant cortices at P7.

<p>(<b>A–D</b>) Enlarged luminal size as well as increased Desmin (in red) expression along cortical vessels (IB4 staining, in green) in mutants (<b>A</b> & <b>C</b>) as compared to controls (<b>B</b> & <b>D</b>) at P7. (<b>E</b>) Quantification of vessel luminal size at P7. Average luminal size in mutants was more than doubled (205%) than in controls (control, 8.28±0.22 μm; mutant, 17.05±0.67 μm; <i>P</i> = 1.4×10⁻¹³, n = 25). (<b>F</b>) Quantification of Desmin positive pericyte fiber density at P7. The average number of continuous Desmin positive fiber per unit vessel length was more than doubled (247%) in mutants than in controls (control, 1.76±0.22/mm; mutant, 4.35±0.23/mm; <i>P</i> = 0.002, n = 3). (<b>G–H</b>) Increased Ki67 (in red) positive endothelial cells (IB4 staining, in green) in mutants (<b>H</b>) as compared to controls (<b>G</b>) at P7. (<b>I–J</b>) Similar levels of Glut-1 (in red) expression along cortical vessels between controls (<b>I</b>) and mutants (<b>J</b>) at P7. (<b>K–L</b>) Quantitative analysis shows no significant changes in the density of Ki67 positive endothelial cells along vessels at P5 (control, 25.2±1.1/cm; mutant, 24.2±2.4/cm; <i>P</i> = 0.69, n = 12) (<b>K</b>), but a 100% increase in mutants at P7 (control, 18.2±0.6/cm; mutant, 36.4±4.9/cm; <i>P</i> = 0.002, n = 13) (<b>L</b>). Scale bar in (<b>A</b>), 50 μm for (<b>A–J</b>).