<i>Baf60c</i> is sufficient to induce smooth muscle like fate in rMAPCs.

<p>rMAPCs were transduced with an inducible <i>Baf60c</i> and eGFP cDNA encoding lentiviral vector or a vector encoding only eGFP, and subjected to SMCs without exogenous TGFβ1, and differentiation to SMCs examined by RT-qPCR and immunostaining. <b>A</b>. Expression of smooth muscle specific mRNAs compared to undifferentiated rMAPC (expression normalized to GAPDH) (Mean±s.e.m of n = 4; p<0.05). <b>B</b>. The expression of <i>αSma</i> in <i>Baf60c</i> transduced cells on day 9 cells is significantly higher compared to GFP transduced control cells (Mean±s.e.m of n = 4; Student's t-test p<0.05). <b>C</b>. <i>Baf60c</i> overexpression is sufficient to induce smooth muscle like fate with cells expressing mature smooth muscle proteins as compared to GFP transduced cells (smooth muscle proteins are colored pseudo green).