Protein import into <i>Chlamydomonas</i> chloroplasts is not differentially regulated.

<p>[³⁵S]Met-labeled precursors of Cr-prRBCS, Cr-prL11, prOE23, prTic40, and prL11 were imported into chloroplasts isolated from synchronized cultures of <i>Chlamydomonas</i> 1, 6, and 11 h after the start of the third light cycle and used for import experiments. Samples were analyzed by SDS-PAGE and autoradiography. An equal number of chloroplasts were loaded in each lane of the same gel. The amount of <i>Chlamydomonas</i> chloroplast Hsp70B was analyzed by immunoblotting and used for normalization of quantifications in the bar graph shown below each gel. The amount of mature protein imported at hour 1 was set as 100%. Data shown are mean ± SD, <i>n</i> = 3. m, mature form; p, precursor form; TR, in vitro–translated precursor proteins before import.