SEPT9 regulates p114GEF and Src/Vav2/RhoA pathway to modulate EC proliferation.

<p>ECs were seeded on HSG and LSG for 24 h. (A) The total cell lysates were immunoblotted with antibodies against p114GEF and SEPT9 (upper panel) and immunoprecipitated with an antibody against SEPT9 followed by immunoblotting with antibodies against p114GEF and SEPT9 (lower panel). (B–C) ECs transfected with control-shRNA or sh-SEPT9 were seeded on HSG and LSG for 24 h, and the cell lysates were immunoblotted with antibodies against phospho-Src (Y416), Src, phospho-Vav2 (Y172), and Vav2 (B) and hyper-phosphorylated Rb, cyclin A, and p27 (C). Bar graph represents the quantified results of three independent experiments. (D) ECs transfected with control-shRNA or sh-SEPT9 were subjected to flow cytometry for cell cycle analyses. * p<0.05 in comparison with the corresponding controls on HSG and LSG. # p<0.05 for comparison between control and sh-SEPT9 on LSG (n = 3).