Dose response curves of MB and derivatives in the HT-22 glutamate model.

<p>(A) Dose response curves of MB and derivative against glutamate-induced neurotoxicity measured by Calcein AM; (B) Dose response curves of MB and derivatives against glutamate-induced cellular ROS production measured by DCF assay; (C) Dose response curves of MB and derivatives against mitochondria membrane potential depolarization induced by glutamate measured by NAO/TMRE FRET assay; (D) Correlation of cellular ROS production and cell viability, Pearson coefficient = 0.8690, p = 0.00111; (E) Correlation of mitochondria membrane potential and cell viability, Pearson coefficient = 0.9456, p = 0.0013; (F) Correlation of cellular ROS production and mitochondria membrane potential, Pearson coefficient = 0.7902, p = 0.0345.