IFN-γ, IL-2, TNF and CD107a responses to the vaccine insert measured by flow cytometry.

<p>Production of IFN-γ (A), IL-2 (B) and TNF (C), and mobilization of CD107a (D), after background subtraction in CD3⁺CD4⁺ (black circles) and CD3⁺CD8⁺ (white circles) cell populations stimulated with a single pool of peptides spanning the complete NP+M1 vaccine insert. Volunteers in group 3 were tested at weeks 0, 1, and 3. Significant differences between pre- and post-vaccination time points were detected using the Wilcoxon signed rank test as follows: IFN-γ CD4⁺, week 1 (p = 0·0001) and week 3 (p = 0·0001); IFN-γ CD8⁺, week 1 (p = 0·001) and week 3 (p = 0·0005); IL-2 CD4⁺, week 1 (p = 0.001) and week 3 (p = 0·0001); IL-2 CD8⁺, week 1 (p = 0·006) and week 3 (p = 0·03); TNF CD4⁺, week 1 (p = 0·002) and week 3 (p = 0·0003); TNF CD8⁺, week 1 (p = 0·0003) and week 3 (p = 0·002); CD107a CD8⁺, week 3 (p = 0·004).