Analysis of apoptosis induced by MVA-C-ΔF1L deletion mutant.

<p>(A) PARP cleavage was analyzed by Western-blot in HeLa cells mock-infected or infected with MVA wt, MVA-C or MVA-C-ΔF1L at 5 PFU/cell for 16 hours. Detection of cellular β-actin protein was used as an internal loading control. (B) Annexin V binding assay of HeLa, 3T3 cells or murine DCs infected with MVA wt, MVA-C or MVA-C-ΔF1L at 5 PFU/cell. At 16 hours post-infection, the infected cells were stained with Annexin V and propidium iodide as described under Materials and Methods and the percentages of early apoptotic cells (Annexin V positive, PI negative) were determined by flow cytometry. All data are mock-infected cells-subtracted. HeLa or 3T3 cells treated with staurosporine (0.5 µM) were used as positive control (not shown). *** <i>p</i><0.001. <i>p</i> value indicates significantly higher response compared to MVA wt or MVA-C-infected cells.